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Immunohistochemical screening for antibodies in recent onset type 1 narcolepsy and after H1N1 vaccination

Published:April 28, 2015DOI:https://doi.org/10.1016/j.jneuroim.2015.04.008
Immunohistochemical screening for antibodies in recent onset type 1 narcolepsy and after H1N1 vaccination
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      Highlights

      • •
        Narcolepsy type 1 is hypothesised to be an autoimmune disease.
      • •
        Screening for antibodies against hypocretin neurons using immunohistochemistry
      • •
        Serum of 21 narcolepsy type 1 patients close to disease onset
      • •
        No antibodies against hypocretin neurons could be detected.

      Abstract

      Narcolepsy type 1 patients typically have undetectable hypocretin-1 levels in the cerebrospinal fluid (CSF), as a result of a selective loss of the hypocretin containing neurons in the hypothalamus. An autoimmune attack targeting hypothalamic hypocretin (orexin) neurons is hypothesised. So far, no direct evidence for an autoimmune attack was found. One of the major limitations of previous studies was that none included patients close to disease onset. We screened serum of 21 narcolepsy type 1 patients close to disease onset (median 11 months), including 8 H1N1 vaccinated patients, for antibodies against hypocretin neurons using immunohistochemistry. No autoantibodies against hypocretin neurons could be detected.

      Keywords

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      Article info

      Publication history

      Published online: April 28, 2015
      Accepted: April 13, 2015
      Received in revised form: March 5, 2015
      Received: January 12, 2015

      Footnotes

      ☆Declaration of interest

      ☆Dr. Lammers has served as a paid member of the UCB advisory board and received lecture fees and conference travel support from UCB pharma. Dr. Lammers also did consultancy for UCB pharma and provided expert testimony for UCB pharma, Jazz pharmaceuticals, and Bioprojet. The other authors report no financial conflict of interest.

      Identification

      DOI: https://doi.org/10.1016/j.jneuroim.2015.04.008

      Copyright

      © 2015 Elsevier B.V. Published by Elsevier Inc. All rights reserved.

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