Abstract
The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of
resident CNS cells, selectively expressing the mature macrophage marker ED2 in the
rat. In order to elucidate the role of the PVM and MM in rats during normal functioning
of the brain and pathology, we have developed a strategy employing a single intraventricular
injection of clodronate liposomes. This resulted in a complete depletion of the PVM
and MM. Clodronate liposomes did not deplete the microglial cells. In other parts
of the body, a temporal and mild depletion effect was observed, which was restored
within 1 week. Detailed analysis of the elimination and repopulation kinetics of the
PVM and MM revealed a slow repopulation of the CNS, starting at 14 days post depletion.
This selective depletion method of the PVM and MM will enable us to get direct insight
in their functions during normal and pathologic conditions of the CNS.
Keywords
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Article info
Publication history
Accepted:
February 16,
2001
Received in revised form:
February 16,
2001
Received:
December 20,
2000
Identification
Copyright
© 2001 Published by Elsevier Inc.
