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Possible role of oxidative stress and immunological activation in mouse model of chronic fatigue syndrome and its attenuation by olive extract

  • Amit Gupta
    Affiliations
    Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study, Panjab University, Chandigarh 160014, India
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  • Garima Vij
    Affiliations
    Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study, Panjab University, Chandigarh 160014, India
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  • Kanwaljit Chopra
    Correspondence
    Corresponding author. Tel.: +91 172 2534105; fax: +91 172 2541142.
    Affiliations
    Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study, Panjab University, Chandigarh 160014, India
    Search for articles by this author

      Abstract

      Various putative theories involved in the development of chronic fatigue syndrome revolve around the role of stress, infection and oxidative stress. Scientific evidence highlighting the protective role of nutritional supplements in chronic fatigue syndrome is lacking. Based on these assumptions, the present study was designed to evaluate the effect of olive extract in a mouse model of immunologically-induced fatigue, wherein purified lipopolysaccharide (LPS) and Brucella abortus (BA) antigen were used as immunogens. The assessment of chronic fatigue syndrome was based on immobility period during chronic water-immersion stress test for 10 min daily. The stress-induced hyperalgesia was measured by tail withdrawal latency. Mice challenged with LPS or BA for 19 days showed significant increase in the immobility time, hyperalgesia and oxidative stress on the 19th day. Serum tumor necrosis factor-alpha (TNF-α) levels were also markedly increased with LPS or BA challenge. Concurrent treatment with olive extract resulted in a significant decrease in the immobility time as well as hyperalgesia. There was significant attenuation of oxidative stress as well as serum TNF-α levels. The results of the present study strongly indicate the role of oxidative stress and immunological activation in the pathophysiology of chronic fatigue syndrome and highlight the valuable role of olive extract in combating chronic fatigue syndrome.

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      References

        • Behan P.O.
        • Behan W.M.
        Postviral fatigue syndrome.
        Crit. Rev. Neurobiol. 1988; 4: 157-158
        • Buchwald D.
        • Wener M.H.
        • Pearlman T.
        • Kith P.
        Markers of inflammation and immune activation in chronic fatigue and chronic fatigue syndrome.
        J. Rheumatol. 1997; 24: 372-376
        • Chao C.C.
        • DeLaHunt M.
        • Hu S.
        • Close K.
        • Peterson P.K.
        Immunologically mediated fatigue: a murine model.
        Clin. Immunol. Immunopathol. 1992; 64: 161-165
        • Chen R.
        • Moriya J.
        • Yamakawa J.I.
        • Takahashi T.
        • Li Q.
        • Morimoto S.
        • Iwai K.
        • Sumino H.
        • Yamaguchi N.
        • Kanda T.
        Brain atrophy in a murine model of chronic fatigue syndrome and beneficial effect of Hochu-ekki-to (TJ-41).
        Neurochem. Res. 2008; 33: 1759-1767
        • Demitrack M.A.
        • Dale J.K.
        • Straus S.E.
        • Laue L.
        • Listwak S.J.
        • Kruesi M.J.
        • Chrousos G.P.
        • Gold P.W.
        Evidence for impaired activation of the hypothalamic–pituitary–adrenal axis in patients with chronic fatigue syndrome.
        J. Clin. Endocrinol. Metab. 1991; 73: 1224-1234
        • Elenkov I.J.
        • Wilder R.L.
        • Chrousos G.P.
        • Vizi E.S.
        The sympathetic nerve—an integrative interface between two supersystems: the brain and the immune system.
        Pharmacol. Rev. 2000; 52: 595-638
        • Ellman G.L.
        Tissue sulfhydryl groups.
        Arch. Biochem. Biophys. 1959; 82: 70-77
        • Ferreira L.F.
        • Reid M.B.
        Muscle-derived ROS and thiol regulation in muscle fatigue.
        J. Appl. Physiol. 2008; 104: 853-860
        • Fukuda K.
        • Straus S.E.
        • Hickie I.
        • Sharpe M.C.
        • Dobbins J.G.
        • Komaroff A.
        The chronic fatigue syndrome: a comprehensive approach to its definition and study. International Chronic Fatigue Syndrome Study Group.
        Ann. Intern. Med. 1994; 121: 953-959
        • Gaab J.
        • Rohleder N.
        • Heitz V.
        • Engert V.
        • Schad T.
        • Schürmeyer T.H.
        • Ehlert U.
        Stress-induced changes in LPS-induced pro-inflammatory cytokine production in chronic fatigue syndrome.
        Psychoneuroendocrinology. 2005; 30: 188-198
        • Goya L.
        • Mateos R.
        • Bravo L.
        Effect of the olive oil phenol hydroxytyrosol on human hepatoma HepG2 cells. Protection against oxidative stress induced by tert-butylhydroperoxide.
        Eur. J. Nutr. 2007; 46: 70-78
        • Gupta A.
        • Chander V.
        • Sharma S.
        • Chopra K.
        Sodium nitroprusside and l-arginine attenuates ferric nitrilotriacetate-induced oxidative renal injury in rats.
        Toxicology. 2007; 232: 183-191
        • Gupta A.
        • Vij G.
        • Sharma S.
        • Tirkey N.
        • Rishi P.
        • Chopra K.
        Curcumin, a polyphenolic antioxidant, attenuates chronic fatigue syndrome in murine water immersion stress model.
        Immunobiology. 2009; 214: 33-39
        • Klimas N.G.
        • Koneru A.O.
        Chronic fatigue syndrome: inflammation, immune function and neuroendocrine interactions.
        Curr. Rheumatol. Rep. 2007; 9: 482-487
        • Lerner A.M.
        • Beqaj S.H.
        • Deeter R.G.
        • Fitzgerald J.T.
        IgM serum antibodies to Epstein–Barr virus are uniquely present in a subset of patients with the chronic fatigue syndrome.
        In Vivo. 2004; 18: 101-106
        • Logan A.C.
        • Wong C.
        Chronic fatigue syndrome: oxidative stress and dietary modifications.
        Altern. Med. Rev. 2001; 6: 450-459
        • MacDonald K.L.
        • Osterholm M.T.
        • LeDell K.H.
        • White K.E.
        • Schenck C.H.
        • Chao C.C.
        • Persing D.H.
        • Johnson R.C.
        • Barker J.M.
        • Peterson P.K.
        A case–control study to assess possible triggers and cofactors in chronic fatigue syndrome.
        Am. J. Med. 1996; 100: 548-554
        • Maes M.
        • Mihaylova I.
        • Kubera M.
        • Bosmans E.
        Not in the mind but in the cell: increased production of cyclo-oxygenase-2 and inducible NO synthase in chronic fatigue syndrome.
        Neuro Endocrinol. Lett. 2007; 28: 463-469
        • Maiuri M.C.
        • De Stefano D.
        • Di Meglio P.
        • Irace C.
        • Savarese M.
        • Sacchi R.
        • Cinelli M.P.
        • Carnuccio R.
        Hydroxytyrosol, a phenolic compound from virgin olive oil, prevents macrophage activation.
        Naunyn. Schmiedebergs Arch. Pharmacol. 2005; 371: 457-465
        • Morganti-Kossmann M.C.
        • Rancan M.
        • Stahel P.F.
        • Kossmann T.
        Inflammatory response in acute traumatic brain injury: a double-edged sword.
        Curr. Opin. Crit. Care. 2002; 8: 101-105
        • Ottenweller J.E.
        • Natelson B.H.
        • Gause W.C.
        • Carroll K.K.
        • Beldowicz D.
        • Zhou X.D.
        • LaManca J.J.
        Mouse running activity is lowered by Brucella abortus treatment: a potential model to study chronic fatigue.
        Physiol. Behav. 1998; 63: 795-801
        • Pall M.L.
        • Satterlee J.D.
        Elevated nitric oxide/peroxynitrite mechanism for the common etiology of multiple chemical sensitivity, chronic fatigue syndrome, and posttraumatic stress disorder.
        Ann. NY Acad. Sci. 2001; 933: 323-329
        • Quan N.
        • Herkenham M.
        Connecting cytokines and brain: a review of current issues.
        Histol. Histopathol. 2002; 17: 273-288
        • Quan N.
        • Stern E.L.
        • Whiteside M.B.
        • Herkenham M.
        Induction of pro-inflammatory cytokine mRNAs in the brain after peripheral injection of subseptic doses of lipopolysaccharide in the rat.
        J. Neuroimmunol. 1999; 93: 72-80
        • Rahman I.
        • Gilmour P.S.
        • Jimenez L.A.
        • MacNee W.
        Oxidative stress and TNF-alpha induce histone acetylation and NF-kappaB/AP-1 activation in alveolar epithelial cells: potential mechanism in gene transcription in lung inflammation.
        Mol. Cell. Biochem. 2002; 234–235: 239-248
        • Reeves W.C.
        • Wagner D.
        • Nisenbaum R.
        Chronic fatigue syndrome—a clinically empirical approach to its definition and study.
        BMC Med. 2005; 3: 19
        • Rietjens S.J.
        • Bast A.
        • Haenen G.R.
        New insights into controversies on the antioxidant potential of the olive oil antioxidant hydroxytyrosol.
        J. Agric. Food Chem. 2007; 55: 7609-76014
        • Sakaguchi S.
        • Furusawa S.
        Oxidative stress and septic shock: metabolic aspects of oxygen-derived free radicals generated in the liver during endotoxemia.
        FEMS Immunol. Med. Microbiol. 2006; 47: 167-177
        • Schaffer S.
        • Podstawa M.
        • Visioli F.
        • Bogani P.
        • Muller W.E.
        • Eckert G.P.
        Hydroxytyrosol-rich olive mill wastewater extract protects brain cells in vitro and ex vivo.
        J. Agric. Food Chem. 2007; 55: 5043-5049
        • Singal A.
        • Kaur S.
        • Tirkey N.
        • Chopra K.
        Green tea extract and catechin ameliorate chronic fatigue-induced oxidative stress in mice.
        J. Med. Food. 2005; 8: 47-52
        • Singh A.
        • Naidu P.S.
        • Gupta S.
        • Kulkarni S.K.
        Effect of natural and synthetic antioxidants in a mouse model of chronic fatigue syndrome.
        J. Med. Food. 2002; 5: 211-220
        • Sugino K.
        • Dohi K.
        • Yamada K.
        • Kawasaki T.
        The role of lipid peroxidation in endotoxin-induced hepatic damage and the protective effect of antioxidants.
        Surgery. 1987; 101: 746-752
        • Svetic A.
        • Jian Y.C.
        • Lu P.
        • Finkelman F.D.
        • Gause W.C.
        Brucella abortus induces a novel cytokine gene expression pattern characterized by elevated IL-10 and IFN-gamma in CD4+ T cells.
        Int. Immunol. 1993; 5: 877-883
        • Vij G.
        • Gupta A.
        • Chopra K.
        Modulation of antigen-induced chronic fatigue in mouse model of water immersion stress by naringin, a polyphenolic antioxidant.
        Fundam. Clin. Pharmacol. 2009; 23: 331-337
        • Visioli F.
        • Caruso D.
        • Galli C.
        • Viappini S.
        • Galli G.
        • Sala A.
        Olive oils rich in natural catecholic phenols decrease isoprostane excretion in humans.
        Biochem. Biophys. Res. Commun. 2000; 278: 797-799
        • Visioli F.
        • Galli C.
        • Plasmati E.
        • Viappiani S.
        • Hernandez A.
        • Colombo C.
        • Sala A.
        Olive phenol hydroxytyrosol prevents passive smoking-induced oxidative stress.
        Circulation. 2000; 102: 2169-2171
        • Wills E.D.
        Mechanisms of lipid peroxide formation in animal tissues.
        Biochem. J. 1966; 99: 667-676
        • Yoshikawa Y.
        • Takano H.
        • Takahashi S.
        • Ichikawa H.
        Changes in tissue antioxidant enzyme activities and lipid peroxides in endotoxin-induced multiple organ failure.
        Circ. Shock. 1994; 42: 53-58