Investigation of autoantibody profiles for cerebrospinal fluid biomarker discovery in patients with relapsing–remitting multiple sclerosis

Abstract 

Using the UNIarray® marker technology platform, cerebrospinal fluid immunoglobulin G reactivities of 15 controls and 17 RRMS patients against human recombinant proteins were investigated. Patient cerebrospinal fluids were oligoclonal band positive and reactivities were compared to that of sex- and age-matched controls. We hereby aimed at the characterization of autoreactivity in patients with RRMS. Differences in autoreactivities between control and RRMS samples were identified comprising autoantigens identified in this study only and previously reported autoantigens as well. A combination of the 10–15 most significant proteins may be investigated further as autoantigens for diagnostic purposes. Additional investigations may include minimizing the number of proteins used in such diagnostic tests.

Abbreviations: AUC, area under curve, BIND, biomolecular interaction network database, CNPase, 2′, 3′-cyclic nucleotide 3′-phosphodiesterase, CSF, cerebrospinal fluid, EBNA-1, Epstein–Barr nuclear antigen 1, FDR, false discovery rate, HSP, heat shock protein, IMAC, immobilized metal ion affinity chromatography, IgG, immunoglobulin G, KEGG, Kyoto Encyclopedia of Genes and Genomes, MS, multiple sclerosis, MBP, myelin basic protein, MOBP, myelin-associated oligodendrocytic basic protein, MOG, myelin oligodendrocyte glycoprotein, Nf, neurofilaments, OCB, oligoclonal band, OMIN, online Mendelian inheritance in man, ORF, open reading frame, OSP, oligodendrocyte-specific protein, PLP, proteolipid protein, ROC, receiver operator characteristic, RRMS, relapsing–remitting MS, SD, standard deviation, SVM, support vector machines

Keywords: Autoantibody profiling, Biomarker, Cerebrospinal fluid, Multiple sclerosis