LRRK2 is expressed in B-2 but not in B-1 B cells, and downregulated by cellular activation

Kubo, Makoto; Kamiya, Yoshiko; Nagashima, Ryuichi; Maekawa, Tatsunori; Eshima, Koji; Azuma, Sadahiro; Ohta, Etsuro; Obata, Fumiya

doi:10.1016/j.jneuroim.2010.07.021

« Previous Next »Journal of Neuroimmunology
Volume 229, Issue 1 , Pages 123-128, 15 December 2010

LRRK2 is expressed in B-2 but not in B-1 B cells, and downregulated by cellular activation

Makoto Kubo
- Division of Clinical Immunology, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
Yoshiko Kamiya
- Division of Clinical Immunology, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
Ryuichi Nagashima
- Division of Clinical Immunology, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
Tatsunori Maekawa
- Division of Clinical Immunology, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
Koji Eshima
- Department of Immunology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
Sadahiro Azuma
- Department of Laboratory Animal Science, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
Etsuro Ohta
- Division of Clinical Immunology, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
Fumiya Obata
- Division of Clinical Immunology, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
- R & D Center for Cell Design, Institute for Regenerative Medicine and Cell Design, Kitasato University School of Allied Health Sciences, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
- Corresponding author. Division of Immunology, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan. Tel.: +81 42 778 8215; fax: +81 42 778 8075.

Received 19 May 2010; received in revised form 22 July 2010; accepted 23 July 2010. published online 23 August 2010.

Abstract

LRRK2, the causal molecule of familial Parkinson's disease, is expressed strongly by one of the B cell subsets, B-2 cells, but not by the other subset, B-1 cells, in the mouse peritoneal cavity, spleen, and peripheral blood. Bone marrow pre-B cells or T cells exhibited little LRRK2 expression. LRRK2 expression was dramatically downregulated upon activation of B-2 cells with various types of stimulation. These results suggest that LRRK2, whose true function has not yet been clarified, may play some important role(s) in the development and function of B cells, particularly the maintenance of B-2 cells in a resting status.

Keywords: Leucine-rich repeat kinase 2, B-1 cells, B-2 cells, Parkinson's disease, PARK8, Downregulation