Qualitative and quantitative evidence of anti-glutamic acid decarboxylase-specific intrathecal antibody synthesis in patients with stiff person syndrome

Jarius, S.; Stich, O.; Speck, J.; Rasiah, Ch.; Wildemann, B.; Meinck, H.M.; Rauer, S.

doi:10.1016/j.jneuroim.2010.07.019

« Previous Next »Journal of Neuroimmunology
Volume 229, Issue 1 , Pages 219-224, 15 December 2010

Qualitative and quantitative evidence of anti-glutamic acid decarboxylase-specific intrathecal antibody synthesis in patients with stiff person syndrome

S. Jarius
- Division of Clinical Neuroimmunology, Department of Neurology, University of Heidelberg, Heidelberg, Germany
- Contributed equally.
O. Stich
- Department of Neurology, Albert Ludwigs University, Freiburg, Germany
- Contributed equally.
J. Speck
- Department of Neurology, Albert Ludwigs University, Freiburg, Germany
Ch. Rasiah
- ravo Diagnostika GmbH, Oltmannsstr. 5, D-79100 Freiburg, Germany
B. Wildemann
- Division of Clinical Neuroimmunology, Department of Neurology, University of Heidelberg, Heidelberg, Germany
H.M. Meinck
- Division of Clinical Neurophysiology, Department of Neurology, University of Heidelberg, Heidelberg, Germany
S. Rauer
- Department of Neurology, Albert Ludwigs University, Freiburg, Germany
- ravo Diagnostika GmbH, Oltmannsstr. 5, D-79100 Freiburg, Germany
- Corresponding author. Department of Neurology, Albert Ludwigs University, Freiburg, Breisacher Strasse 64, D-79106 Freiburg, Germany. Tel.: +49 761 270 5001; fax: +49 761 270 5024.

Received 20 November 2009; received in revised form 7 May 2010; accepted 22 July 2010. published online 03 September 2010.

Abstract

Background

The stiff person syndrome (SPS) is a CNS disorder of putative autoimmune aetiology, which is clinically characterized by severe rigidity and spasms. In most cases, SPS is associated with serum antibodies against glutamic acid decarboxylase (GAD-Ab). Recent studies suggested that GAD-Ab might be directly involved in the pathogenesis of SPS. Further support for this hypothesis would come from studies providing qualitative evidence for the presence of GAD-Ab-producing B cell clones within the CNS of patients with SPS.

Objective and methods

To address that issue, we (i) analysed paired cerebrospinal fluid (CSF) and serum samples from ten GAD-Ab positive patients with SPS and controls by an antigen-driven affinity blotting technique for the presence of GAD-specific oligoclonal IgG bands (OCBs) in the CSF, and (ii) examined the immunoreactive pattern of CSF and serum IgG to recombinant GAD by immunoblotting. To confirm our results quantitatively, we (iii) assessed anti-GAD antibody reactivity in CSF and serum using ELISA and evaluated the GAD-specific antibody index.

Results

GAD-specific oligoclonal bands exclusively or predominately in CSF compared to the corresponding serum were detected in 10/10 patients with GAD-positive SPS but in none of the controls. Immunoblotting revealed stronger staining in the CSF, suggestive of intrathecal IgG synthesis, in 7/10 patients upon visual inspection, and in 8/10 patients upon densitometric analysis. A positive GAD-specific antibody index was found in 9/10 patients.

Conclusions

Here we demonstrate for the first time that IgG OCBs in SPS bind GAD. Our findings contribute to the ongoing discussion on whether the autoimmune process against GAD is involved in the pathogenesis of SPS by indicating that anti-GAD-Ab is produced by B cell clones within the CNS.

Keywords: Stiff person syndrome/stiff man syndrome, Glutamic acid decarboxylase (GAD) antibody, Cerebrospinal fluid, Intrathecal antibody synthesis, Antibody index, Specific oligoclonal bands