Expression and regulation in the brain of the chemokine CCL27 gene locus

Gunsolly, Chad; Nicholson, James D.; Listwak, Samuel J.; Ledee, Dolena; Zelenka, Peggy; Verthelyi, Daniela; Chapoval, Svetlana; Keegan, Achsah; Tonelli, Leonardo H.

doi:10.1016/j.jneuroim.2010.04.019

« Previous Next »Journal of Neuroimmunology
Volume 225, Issue 1 , Pages 82-90, 25 August 2010

Expression and regulation in the brain of the chemokine CCL27 gene locus

Chad Gunsolly
- Laboratory of Behavioral Neuroimmunology, Mood and Anxiety Program, Department of Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland, United States
James D. Nicholson
- Laboratory of Behavioral Neuroimmunology, Mood and Anxiety Program, Department of Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland, United States
- Present address: Department of Ophthalmology Research, University of Maryland School of Medicine, Maryland, United States.
Samuel J. Listwak
- National Institute of Mental Health, NIH, Bethesda, Maryland, United States
Dolena Ledee
- Cell Differentiation Section, National Eye Institute, NIH, Bethesda, Maryland, United States
Peggy Zelenka
- Cell Differentiation Section, National Eye Institute, NIH, Bethesda, Maryland, United States
Daniela Verthelyi
- Division of Therapeutic Proteins, Center for Drug Evaluation and Review, Food and Drug Administration, Bethesda, Maryland, United States
Svetlana Chapoval
- Department of Microbiology and Immunology, Center for Vascular and Inflammatory Diseases and Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, United States
Achsah Keegan
- Department of Microbiology and Immunology, Center for Vascular and Inflammatory Diseases and Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, United States
Leonardo H. Tonelli
- Laboratory of Behavioral Neuroimmunology, Mood and Anxiety Program, Department of Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland, United States
- Corresponding author. 685 West Baltimore Street, MSTF Building room 934 C Baltimore, Maryland, 21201-1549, United States. Tel.: +1 410 706 2325; fax: +1 410 706 0751.

Received 14 January 2010; received in revised form 31 March 2010; accepted 28 April 2010. published online 04 June 2010.

Abstract

The chemokine CCL27 has chemoattractant properties for memory T cells and has been implicated in skin allergic reactions. The present study reports the expression in the brain of two CCL27 splice variants localized in the cerebral cortex and limbic regions. CCL27-like immunoreactivity was identified mainly in neurons. Variant 1 was found elevated in the olfactory bulbs during allergic inflammation induced by intranasal challenge with allergen. This was accompanied by the presence of T cells in the olfactory bulbs. Intranasal administration of neutralizing antibodies against CCL27 reduced the presence of T cells in the olfactory bulbs suggesting a function in T cell activity in the brain.

Keywords: CTACK, PESKY, Alternative splicing, In situ hybridization, Neuroimmune, RT-PCR