Journal of Neuroimmunology
Volume 225, Issue 1 , Pages 34-42, 25 August 2010

Cytotoxic T-lymphocytes secrete soluble factors that induce caspase-mediated apoptosis in glioblastoma cell lines

  • Alberto Cagigi

      Affiliations

    • Department of Laboratory Medicine, Division of Clinical Immunology, Karolinska University Hospital Huddinge, Alfred Nobels Allé 8, S 141 86 Stockholm, Sweden
    • Corresponding Author InformationCorresponding author. Tel.: +46 0046739926078.
    • Equally contributing authors.
    • ,
  • Anna Nilsson

      Affiliations

    • Dept of Women and Child Health, Karolinska Institutet, Astrid Lindgrens Children Hospital, Stockholm, Sweden
    • Equally contributing authors.
    • ,
  • Victor Levitsky

      Affiliations

    • Oncology Department, Johns Hopkins University School of Medicine, Baltimore, USA
    • ,
  • Farideh Sabri

      Affiliations

    • Institute of Environmental Medicine, Division of Toxicology and Neurotoxicology, Karolinska Institutet, Stockholm, Sweden

Received 22 December 2009; received in revised form 13 April 2010; accepted 14 April 2010. published online 18 May 2010.

Abstract 

We have previously shown that factors secreted by activated CTLs induce apoptosis in a panel of glioblastoma lines. In this study, we analyzed the expression of death receptors, activation of caspases and mRNA expression of 96 apoptotic genes in glioblastoma lines either sensitive or resistant to supernatant of activated CTLs. Our results indicate that exposure to supernatant triggers several pathways of caspase activation in glioblastoma lines involved in the initiation of both extrinsic and intrinsic apoptosis. High steady-state levels of Bcl-2 were identified as potentially accounting for the resistance of a proportion of glioblastoma lines to factors secreted by activated CTLs.

Keywords: Astrocytoma, Apoptosis, CTL supernatant, Caspase 2, Microarray

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PII: S0165-5728(10)00169-4

doi:10.1016/j.jneuroim.2010.04.009

Journal of Neuroimmunology
Volume 225, Issue 1 , Pages 34-42, 25 August 2010

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