IFN-β reverses the lipopolysaccharide-induced proteome modifications in treated astrocytes

Vergara, Daniele; Martignago, Roberta; Bonsegna, Stefania; De Nuccio, Francesco; Santino, Angelo; Nicolardi, Giuseppe; Maffia, Michele

doi:10.1016/j.jneuroim.2010.01.002

« Previous Next »Journal of Neuroimmunology
Volume 221, Issue 1 , Pages 115-120, 15 April 2010

IFN-β reverses the lipopolysaccharide-induced proteome modifications in treated astrocytes

Daniele Vergara
- Laboratory of General Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 73100, Lecce, Italy
Roberta Martignago
- Laboratory of Anatomy, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 73100, Lecce, Italy
Stefania Bonsegna
- Institute of Sciences of Food Production CNR-ISPA, Unit of Lecce, Italy
Francesco De Nuccio
- Laboratory of Anatomy, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 73100, Lecce, Italy
Angelo Santino
- Institute of Sciences of Food Production CNR-ISPA, Unit of Lecce, Italy
Giuseppe Nicolardi
- Laboratory of Anatomy, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 73100, Lecce, Italy
Michele Maffia
- Laboratory of General Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 73100, Lecce, Italy
- Laboratory of Clinical Proteomic, “Vito Fazzi” Hospital, ASL-Lecce, Italy
- Corresponding author. Laboratory of General Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 73100, Lecce, Italy. Fax: +39 0832 324200.

Received 15 November 2009; received in revised form 5 January 2010; accepted 5 January 2010. published online 01 February 2010.

Abstract

Astrocytes have a key role in the pathogenesis of several diseases, including multiple sclerosis, and are proposed as a possible target for immunotherapy.

Our earlier study reported that astrocytes treated with IFN-β modified their biomechanical properties possibly due to changes in the expression of the proteins involved in cytoskeleton organization and other important physiological processes.

To gain insight into the mechanism underlying IFN-β action during inflammation, we stimulated astrocytes with LPS, a bacterial wall component used as a model for both in vitro and in vivo immunological stimulation of microglia and astrocytes.

We showed that IFN-β reverses the effects of LPS on the proteome of astrocytes. To better examine this result, we performed a proteomic analysis of astrocytes treated with LPS or LPS plus IFN-β. Treatment with LPS caused increases both in a series of proteins mainly involved in cytoskeletal changes and in protein degradation, as well as protective enzymes like superoxide dismutase. IFN-β reverses LPS effects on astrocyte proteome, supporting its protective role during inflammatory insults.

Keywords: Astrocytes, Interferon-β, Lipopolysaccharide, Multiple sclerosis, 2-DE