Peroxisome proliferator-activated receptor-γ agonists suppress iNOS expression induced by LPS in rat primary Schwann cells

Zhang, Fupeng; Liu, Fen; Yan, Meijuan; Ji, Huoyan; Hu, Ling; Li, Xiaohong; Qian, Ji; He, Xingxin; Zhang, Li; Shen, Aiguo; Cheng, Chun

doi:10.1016/j.jneuroim.2009.10.016

« Previous Next »Journal of Neuroimmunology
Volume 218, Issue 1 , Pages 36-47, 25 January 2010

Peroxisome proliferator-activated receptor-γ agonists suppress iNOS expression induced by LPS in rat primary Schwann cells

Fupeng Zhang
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
- Both authors contributed equally to this work.
Fen Liu
- Laboratory of Cellular and Molecular Tumor Immunology, Cyrus Tang Hematology Center, Medical College of Soochow University, Suzhou, 215123, China
- Both authors contributed equally to this work.
Meijuan Yan
- Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Huoyan Ji
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Ling Hu
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Xiaohong Li
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Ji Qian
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Xingxin He
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Li Zhang
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Aiguo Shen
- Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, 226001, China
- Corresponding authors. Shen is to be contacted at Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, China, 226001. Tel./fax: +86 513 85051999. Cheng, Department of Microbiology and Immunology, Medical College, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, China, 226001. Tel./fax: +86 513 85051999.
Chun Cheng
- Department of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
- Corresponding authors. Shen is to be contacted at Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, China, 226001. Tel./fax: +86 513 85051999. Cheng, Department of Microbiology and Immunology, Medical College, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, China, 226001. Tel./fax: +86 513 85051999.

Received 2 June 2009; received in revised form 6 October 2009; accepted 22 October 2009. published online 27 November 2009.

Abstract

In bacterial-induced peripheral nervous system (PNS) inflammation, Schwann cells (SCs) are activated, producing inducible nitric oxide synthase (iNOS), contributed to the pathogenesis of demyelinating disease, such as multiple sclerosis. Peroxisome proliferator-activated receptor-gamma (PPAR-γ) has been shown to play a protective role in cellular inflammatory responses. Here we showed that LPS-induced iNOS biosynthesis was in a concentration and time-dependent manner. In LPS-treated primary SCs, retreatment with PPAR-γ agonist remitted the increase of iNOS, p38 phosphorylation and TLR4, MyD88, augmented the expression of PPAR-γ and localization in nuclear. Coadministration of GW 9662 reversed the effect of PPAR-γ agonists. These results suggest that PPAR-γ agonists, 15d-PGJ₂ and pioglitazone, had the anti-inflammatory effects.

Keywords: Schwann cell (SC), Lipopolysaccharide, Mitogen-activated protein kinase, Peroxisome proliferators-activated receptor γ (PPAR-γ), Inflammation