Peroxisome proliferator-activated receptor-γ agonists suppress iNOS expression induced by LPS in rat primary Schwann cells
Received 2 June 2009; received in revised form 6 October 2009; accepted 22 October 2009. published online 27 November 2009.
Abstract
In bacterial-induced peripheral nervous system (PNS) inflammation, Schwann cells (SCs) are activated, producing inducible nitric oxide synthase (iNOS), contributed to the pathogenesis of demyelinating disease, such as multiple sclerosis. Peroxisome proliferator-activated receptor-gamma (PPAR-γ) has been shown to play a protective role in cellular inflammatory responses. Here we showed that LPS-induced iNOS biosynthesis was in a concentration and time-dependent manner. In LPS-treated primary SCs, retreatment with PPAR-γ agonist remitted the increase of iNOS, p38 phosphorylation and TLR4, MyD88, augmented the expression of PPAR-γ and localization in nuclear. Coadministration of GW 9662 reversed the effect of PPAR-γ agonists. These results suggest that PPAR-γ agonists, 15d-PGJ2 and pioglitazone, had the anti-inflammatory effects.
aDepartment of Microbiology and Immunology, Medical College, Nantong 19 Qixiu Road, Nantong, Jiangsu, 226001, China
bLaboratory of Cellular and Molecular Tumor Immunology, Cyrus Tang Hematology Center, Medical College of Soochow University, Suzhou, 215123, China
cJiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, 226001, China
Corresponding authors. Shen is to be contacted at Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, China, 226001. Tel./fax: +86 513 85051999. Cheng, Department of Microbiology and Immunology, Medical College, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, China, 226001. Tel./fax: +86 513 85051999.
ABSTRACT