Cerebrospinal fluids containing anti-HSP70 autoantibodies from multiple sclerosis patients augment HSP70-induced proinflammatory cytokine production in monocytic cells

Received 28 June 2009; received in revised form 9 October 2009; accepted 13 October 2009. published online 18 November 2009.

Abstract

Anti-heat shock protein 70 (HSP70) autoantibodies are found in cerebrospinal fluids (CSF) from patients with multiple sclerosis. These autoantibodies cross-reacted not only with HSP70 and heat shock cognate protein 70 (HSC70), but also with a bacterial homologue, DnaK. CSF with a high anti-HSP70 autoantibody titer enhanced HSP70-induced proinflammatory cytokine and chemokine production in the human monocytic cell line, THP-1. Thus, anti-HSP70 autoantibodies in the CSF of multiple sclerosis patients may play a pathophysiological role in enhancing inflammation.

Keywords: Multiple sclerosis, Heat shock protein, Autoantibody, Proinflammatory cytokine, Cerebrospinal fluid

a Department of Microbiology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo 060-8556, Japan

b Department of Neurology, Sapporo Yamanoue Hospital, Nishi-ku, Sapporo 063-0006, Japan

Corresponding author. Department of Microbiology, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo 060-8556, Japan. Tel.: +81 11 611 2111x2710; fax: +81 11 612 5861.

PII: S0165-5728(09)00401-9

doi:10.1016/j.jneuroim.2009.10.009

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