Clonally expanded plasma cells in the cerebrospinal fluid of patients with central nervous system autoimmune demyelination produce “oligoclonal bands”

von Büdingen, Hans-Christian; Gulati, Monica; Kuenzle, Sandra; Fischer, Katja; Rupprecht, Tobias A.; Goebels, Norbert

doi:10.1016/j.jneuroim.2009.10.005

« Previous Next »Journal of Neuroimmunology
Volume 218, Issue 1 , Pages 134-139, 25 January 2010

Clonally expanded plasma cells in the cerebrospinal fluid of patients with central nervous system autoimmune demyelination produce “oligoclonal bands”

Hans-Christian von Büdingen
- Dept. of Neurology, University Hospital Zürich, Frauenklinikstrasse 26, 8091 Zürich, Switzerland
- Dept. of Neurology, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
- Corresponding author. Dept. of Neurology, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA. Tel.: +1 415 476 9046; fax: +1 415 476 5229.
- Equal contribution.
Monica Gulati
- Dept. of Neurology, University Hospital Zürich, Frauenklinikstrasse 26, 8091 Zürich, Switzerland
- Equal contribution.
Sandra Kuenzle
- Dept. of Neurology, University Hospital Zürich, Frauenklinikstrasse 26, 8091 Zürich, Switzerland
Katja Fischer
- Dept. of Neurology, University Hospital Zürich, Frauenklinikstrasse 26, 8091 Zürich, Switzerland
Tobias A. Rupprecht
- Dept. of Neurology, University Hospital Zürich, Frauenklinikstrasse 26, 8091 Zürich, Switzerland
- Dept. of Neurology, Ludwig-Maximilians University, Marchioninistr. 15, 81377 Munich, Germany
Norbert Goebels
- Dept. of Neurology, University Hospital Zürich, Frauenklinikstrasse 26, 8091 Zürich, Switzerland
- Dept. of Neurology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland
- Present address: Dept. of Neurology, University of Düsseldorf, Moorenstrasse 5, D-40225 Düsseldorf, Germany

Received 20 July 2009; received in revised form 4 October 2009; accepted 9 October 2009. published online 10 November 2009.

Abstract

Clonally expanded plasma cells (cePC) and oligoclonal IgG (oligoclonal bands, OCB) in the cerebrospinal fluid (CSF) suggest an involvement of B cell mechanisms in autoimmune CNS demyelination. Due to their CSF-restricted occurrence, OCB are commonly believed to be the products of B cells inside the borders of the blood brain barrier. A comparison of CSF cell Ig transcriptomes and CSF-Ig proteomes recently demonstrated, that in multiple sclerosis patients CSF cells are the origin of CSF immunoglobulins. We expand these findings by applying anti-idiotypic antibodies to detect specific heavy chain CDR3 idiotopes of cePC-produced antibodies amongst OCB in the CSF of a patient each with MS and acute disseminated encephalomyelitis.

Abbreviations: MS, multiple sclerosis, CSF, cerebrospinal fluid, OCB, oligoclonal IgG bands, MOG, myelin oligodendrocyte glycoprotein, MBP, myelin basic protein, PLP, proteolipid protein, cePC, clonally expanded plasma cell, rAb, monoclonal antibody derived from cePC HCDR3, immunoglobulin heavy chain variable region complementarity-determining region 3