Journal of Neuroimmunology
Volume 217, Issue 1 , Pages 74-79 , 10 December 2009

Western blot analysis for the detection of serum antibodies recognizing linear Aquaporin-4 epitopes in patients with Neuromyelitis Optica

  • Fabiana Marnetto

      Affiliations

    • SCDO: Neurologia 2, Centro Sclerosi Multipla (CReSM), AOU S. Luigi Gonzaga, Orbassano, Torino, Italy
    • Corresponding Author InformationCorresponding author. SCDO: Neurologia 2, Centro Sclerosi Multipla (CReSM), AOU S. Luigi Gonzaga, Regione Gonzole 10, I-10043, Orbassano, Torino, Italy. Tel.: +39 011 9026437; fax: +39 011 9026397.
    • ,
  • Bianca Hellias

      Affiliations

    • Istituto scientifico Cavalieri Ottolenghi, Università degli studi di Torino, AOU S. Luigi Gonzaga, Orbassano, Torino, Italy
    • ,
  • Letizia Granieri

      Affiliations

    • SCDO: Neurologia 2, Centro Sclerosi Multipla (CReSM), AOU S. Luigi Gonzaga, Orbassano, Torino, Italy
    • ,
  • Jessica Frau

      Affiliations

    • Centro Sclerosi Multipla, Dipartimento di Scienze Neurologiche e Cardiovascolari, Università degli Studi di Cagliari, Cagliari, Italy
    • ,
  • Agata Katia Patanella

      Affiliations

    • Centro Sclerosi Multipla, Dipartimento di Neuroscienze, Policlinico Universitario Agostino Gemelli, UCSC Rome, Italy
    • ,
  • Petra Nytrova

      Affiliations

    • Department of Neurology, Charles University in Prague, 1st Faculty of Medicine and General Teaching Hospital, Prague, Czech Republic
    • ,
  • Arianna Sala

      Affiliations

    • SCDO: Neurologia 2, Centro Sclerosi Multipla (CReSM), AOU S. Luigi Gonzaga, Orbassano, Torino, Italy
    • ,
  • Marco Capobianco

      Affiliations

    • SCDO: Neurologia 2, Centro Sclerosi Multipla (CReSM), AOU S. Luigi Gonzaga, Orbassano, Torino, Italy
    • ,
  • Francesca Gilli

      Affiliations

    • SCDO: Neurologia 2, Centro Sclerosi Multipla (CReSM), AOU S. Luigi Gonzaga, Orbassano, Torino, Italy
    • ,
  • Antonio Bertolotto

      Affiliations

    • SCDO: Neurologia 2, Centro Sclerosi Multipla (CReSM), AOU S. Luigi Gonzaga, Orbassano, Torino, Italy

Received 10 June 2009 ,Revised 29 September 2009 ,Accepted 2 October 2009.

References 

  1. Apiwattanakul M, McKeon A, Fryer JP, Hinson SR, Pittock SJ, Lennon VA. AQP4-IgG immunoprecipitation assay optimization. Clin. Chem. 2009;55(3):592–594
  2. Graber DJ, Levy M, Kerr D, Wade WF. Neuromyelitis optica pathogenesis and aquaporin 4. J. Neuroinflammation. 2008;29(5):22
  3. Hayakawa S, Mori M, Okuta A, Kamegawa A, Fujiyoshi Y, Yoshiyama Y, et al. Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay. J. Neuroimmunol. 2008;30(196(1-2):181–187
  4. Jarius S, Franciotta D, Bergamaschi R, Wright H, Littleton E, Palace J, et al. NMO-IgG in the diagnosis of neuromyelitis optica. Neurology. 2007;27(68(13):1076–1077
  5. Lennon VA, Wingerchuk DM, Kryzer TJ, Pittock SJ, Lucchinetti CF, Fujihara K, et al. A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis. Lancet. 2004;364:2106–2112
  6. Lennon VA, Kryzer TJ, Pittock SJ, Verkman AS, Hinson SR. IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J. Exp. Med. 2005;202:473–477
  7. Ma T, Yang B, Verkman AS. Gene structure, cDNA cloning, and expression of a mouse mercurial-insensitive water channel. Genomics. 1996;33:382–388
  8. Marnetto F., Granieri L., Sala A., Frau J., Patanella AK., Gilli F., Capobianco M., Wandinger KP., Bertolotto A. 2009. Validation of a multi-parametric immunofluorescence assay for the detection of anti-AQP4 antibodies in the diagnosis of Neuromyelitis optica. Multiple Sclerosis 15, S31–S150, P154.
  9. Matiello M., Schaefer-Klein J., Hebrink D., Kingsbury D., Lennon V., Weinshenker B. 2009. Genetic analysis of Neuromyelitis optica: two different Arg19 mutations in the N-terminus of AQP4 suggest a molecular mechanism for susceptibility to NMO. Multiple Sclerosis 15, S31–S150, P262.
  10. Moe SE, Sorbo JG, Sogaard R, Zeuthen T, Petter Ottersen O, Holen T. New isoforms of rat Aquaporin-4. Genomics. 2008;91:367–377
  11. Neely JD, Christensen BM, Nielsen S, Agre P. Heterotetrameric composition of aquaporin-4 water channels. Biochemistry. 1999;38:11156–11163
  12. Nicchia GP, Mastrototaro M, Rossi A, Pisani F, Tortorella C, Ruggeri M, et al. Aquaporin-4 orthogonal arrays of particles are the target for neuromyelitis optica autoantibodies. Glia. 2009;57:1363–1373
  13. Paul F, Jarius S, Aktas O, Bluthner M, Bauer O, Appelhans H, et al. Antibody to aquaporin 4 in the diagnosis of neuromyelitis optica. PLoS Med. 2007;4:e133
  14. Polman CH, Reingold SC, Edan G, Filippi M, Hartung HP, Kappos L, et al. Diagnostic criteria for multiple sclerosis: 2005 revisions to the “McDonald Criteria”. Ann. Neurol. 2005;58(6):840–846
  15. Rash JE, Davidson KG, Yasumura T, Furman CS. Freeze-fracture and immunogold analysis of aquaporin-4 (AQP4) square arrays, with models of AQP4 lattice assembly. Neuroscience. 2004;129:915–934
  16. Takahashi T, Fujihara K, Nakashima I, Misu T, Miyazawa I, Nakamura M, et al. Establishment of a new sensitive assay for anti-human aquaporin-4 antibody in neuromyelitis optica. Tohoku J. Exp. Med. 2006;210:307–313
  17. Takahashi T, Fujihara K, Nakashima I, Misu T, Miyazawa I, Nakamura M, et al. Anti-aquaporin-4 antibody is involved in the pathogenesis of NMO: a study on antibody titre. Brain. 2007;130:1235–1243
  18. Tanaka K, Tani T, Tanaka M, Saida T, Idezuka J, Yamazaki M, et al. Anti-aquaporin 4 antibody in selected Japanese multiple sclerosis patients with long spinal cord lesions. Mult. Scler. 2007;13:850–855
  19. Tani T., Sakimura K., Tsujita M., Nakada T., Tanaka M., Nishizawa M., Tanaka K., 2009. Identification of binding sites for anti-aquaporin 4 antibodies in patients with neuromyelitis optica. J. Neuroimmunol. 25; 211(1–2):110–3.
  20. Verbavatz JM, Ma T, Gobin R, Verkman AS. Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4. J. Cell Sci. 1997;110:2855–2860
  21. Waters P, Vincent A. Detection of anti-aquaporin-4 antibodies in neuromyelitis optica: current status of the assays. Int. MS J. 2008;15(3):99–105
  22. Waters P, Jarius S, Littleton E, Leite MI, Jacob S, Gray B, et al. Aquaporin-4 antibodies in neuromyelitis optica and longitudinally extensive transverse myelitis. Arch. Neurol. 2008;65(7):913–919
  23. Wingerchuk DM, Lennon VA, Pittock SJ, Lucchinetti CF, Weinshenker BG. Revised diagnostic criteria for neuromyelitis optica. Neurology. 2006;23(66(10):1485–1489

PII: S0165-5728(09)00393-2

doi: 10.1016/j.jneuroim.2009.10.002

Journal of Neuroimmunology
Volume 217, Issue 1 , Pages 74-79 , 10 December 2009

Article Tools

* Image Usage & Resolution