Journal of Neuroimmunology
Volume 217, Issue 1 , Pages 46-50, 10 December 2009

Polyclonal immunoglobulins (IVIg) induce expression of MMP-9 in microglia

  • R. Pul

      Affiliations

    • Dept. of Neurology, Hannover Medical School, Hannover, Germany
    • ,
  • T. Kopadze

      Affiliations

    • Dept. of Neurology, Heinrich-Heine-Universität, Düsseldorf, Germany
    • ,
  • T. Skripuletz

      Affiliations

    • Dept. of Neurology, Hannover Medical School, Hannover, Germany
    • ,
  • E.V. Voss

      Affiliations

    • Dept. of Neurology, Hannover Medical School, Hannover, Germany
    • ,
  • B.C. Kieseier

      Affiliations

    • Dept. of Neurology, Heinrich-Heine-Universität, Düsseldorf, Germany
    • ,
  • M. Stangel

      Affiliations

    • Dept. of Neurology, Hannover Medical School, Hannover, Germany
    • Center for Systems Neurosciences, Hannover, Germany
    • Corresponding Author InformationCorresponding author. Dept. of Neurology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Tel.: +49 511 5326676; fax: +49 511 5323115.

Received 12 June 2009; received in revised form 31 August 2009; accepted 23 September 2009. published online 15 October 2009.

Abstract 

In multiple sclerosis (MS) matrix metalloproteinases (MMP) are believed to be involved in the disruption of the blood brain barrier and demyelination. MMP-9 is increased in the cerebrospinal fluid of MS patients and expressed in MS lesions, indicating an involvement in MS pathogenesis. It is known that activated microglia secrete MMP. Modulation of MMP may thus be of interest for treatment in particular since MMP knock-out mice are less susceptible to experimental allergic encephalomyelitis. In this study we show that intact polyclonal immunoglobulins for intravenous use (IVIg) lead to increased secretion of MMP-9 in unstimulated microglia whereas F(ab′)2 fragments or stimulation with lipopolysaccharide (LPS) had no effect on MMP production at all. We could not detect MMP-2, MMP-3, MMP-7, MMP-10, MMP-11, and MMP-12 by RT-PCR with and without stimulation with LPS. IVIg differentially modulate MMP-9 production in resting and activated microglia suggesting an activation-dependent immune response.

Keywords: Microglia, MMP-9, Matrix metalloproteinase, IVIg, Intravenous immunoglobulins

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PII: S0165-5728(09)00384-1

doi:10.1016/j.jneuroim.2009.09.016

Journal of Neuroimmunology
Volume 217, Issue 1 , Pages 46-50, 10 December 2009

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