Journal of Neuroimmunology
Volume 211, Issue 1 , Pages 23-38, 25 June 2009

Strain-dependent inflammatory responsiveness of rat microglial cells

Division of Mental Health and Substance Abuse Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan

Received 1 October 2008; received in revised form 17 February 2009; accepted 5 March 2009. published online 08 April 2009.

Abstract 

The aim of this study is to test whether inflammatory responsiveness of rat microglial cells is strain-specific in primary microglia derived from neonatal LEW/N and F344/N rats. In contrast to F344/N microglia, LEW/N microglia constitutively and upon lipopolysaccharide challenge expressed higher levels of mRNA for the majority of inflammatory mediators studied. In addition, LEW/N microglia exhibited enhanced secretion of tumor necrosis factor-α and CCL2, as well as elevated nitric oxide production. On the contrary, activated LEW/N microglia transcribed and secreted less interleukin-10. Hence, compared to F344/N microglia, LEW/N microglia might be more reactive to lipopolysaccharide and incompetent to suppress inflammation.

Keywords: LEW/N rats, F344/N rats, Pro-inflammatory mediators, IL-10, NADPH oxidase

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PII: S0165-5728(09)00101-5

doi:10.1016/j.jneuroim.2009.03.007

Journal of Neuroimmunology
Volume 211, Issue 1 , Pages 23-38, 25 June 2009

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