Proteome analysis of cerebrospinal fluid in Guillain–Barré syndrome (GBS)

Lehmensiek, V.; Süssmuth, S.D.; Brettschneider, J.; Tauscher, G.; Felk, S.; Gillardon, F.; Tumani, H.

doi:10.1016/j.jneuroim.2007.01.022

« Previous Next »Journal of Neuroimmunology
Volume 185, Issue 1 , Pages 190-194, April 2007

Proteome analysis of cerebrospinal fluid in Guillain–Barré syndrome (GBS)

V. Lehmensiek
- Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany
S.D. Süssmuth
- Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany
J. Brettschneider
- Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany
G. Tauscher
- Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany
S. Felk
- Boehringer Ingelheim Pharma GmbH and Co.KG, CNS Research, 88397 Biberach an der Riss, Germany
F. Gillardon
- Boehringer Ingelheim Pharma GmbH and Co.KG, CNS Research, 88397 Biberach an der Riss, Germany
H. Tumani
- Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany
- Corresponding author. Tel.:+49 731 1775204; fax:+49 731 1771519.

Received 4 October 2006; received in revised form 24 January 2007; accepted 29 January 2007. published online 16 March 2007.

Abstract

We used two-dimensional difference in-gel electrophoresis (2-D-DIGE) for proteome analysis of cerebrospinal fluid (CSF) in Guillain–Barré syndrome (GBS). Spots showing >2-fold difference between GBS and controls were analysed using MALDI-TOF mass spectrometry.

Proteins that were up-regulated in GBS included haptoglobin, serine/threonine kinase 10, α-1-antitrypsin, SNC73, alpha II spectrin, IgG kappa chain and cathepsin D preprotein, while transferrin, caldesmon, GALT, human heat shock protein 70, amyloidosis patient HL-heart-peptide 127aa and transthyretin were down-regulated. Some of these proteins are reported in CSF of GBS for the first time. Accordingly, the 2-D-DIGE technology may be useful to identify disease-specific proteins in patients with GBS.

Keywords: Guillain–Barré syndrome, Proteomics, 2-D-difference in-gel electrophoresis, Cerebrospinal fluid