Journal of Neuroimmunology
Volume 178, Issue 1 , Pages 87-99, September 2006

Modulation of astrocyte proliferation by HIV-1: Differential effects in productively infected, uninfected, and Nef-expressing cells

  • Melissa A. Cosenza-Nashat

      Affiliations

    • Borough of Manhattan Community College, City University of New York, Department of Science, New York, NY 10007, United States
    • ,
  • Qiusheng Si

      Affiliations

    • Department of Pathology F717, Albert Einstein College of Medicine, 1300 Morris Park Avenue Bronx NY 10461, United States
    • ,
  • Meng-Liang Zhao

      Affiliations

    • Department of Pathology F717, Albert Einstein College of Medicine, 1300 Morris Park Avenue Bronx NY 10461, United States
    • ,
  • Sunhee C. Lee

      Affiliations

    • Department of Pathology F717, Albert Einstein College of Medicine, 1300 Morris Park Avenue Bronx NY 10461, United States
    • Corresponding Author InformationCorresponding author. Tel.: +1 718 430 2666; fax: +1 718 430 8867.

Received 23 March 2005; received in revised form 11 April 2006; accepted 16 May 2006. published online 03 July 2006.

Abstract 

Although quiescent in normal brain, reactive astrocytes can proliferate in various disorders. We examined the impact of HIV-1 on astrocyte proliferation in cultures exposed to VSVg env-pseudotyped HIV-1 which yields high levels of infection. HIV-1, while increasing the proliferation of uninfected (p24−) astrocytes, strongly inhibited proliferation of productively infected (p24+) cells. The cell cycle arrest was G1/S rather than G2/M, a type commonly attributed to Vpr. No clear role of Vpr or Nef could be identified. Adenovirus-mediated expression of Nef (a model of “restricted” infection) induced M-phase arrest of astrocytes. We speculate that HIV-1 is a significant modulator of astrocyte proliferation in vivo.

Keywords: Cell cycle arrest, Human, Ki-67, BrdU, Vpr

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PII: S0165-5728(06)00193-7

doi:10.1016/j.jneuroim.2006.05.020

Journal of Neuroimmunology
Volume 178, Issue 1 , Pages 87-99, September 2006

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