Journal of Neuroimmunology
Volume 173, Issue 1 , Pages 166-173, April 2006

Cytokine profile of iron-laden macrophages: Implications for cellular magnetic resonance imaging

  • Ines Siglienti

      Affiliations

    • Department of Neurology, Julius-Maximilians-Universität, Josef-Schneider-Str. 11, 97080 Würzburg, Germany
    • Current address: Institute for MS-Research, Georg-August-Universität and Gemeinnützige Hertie-Stiftung, Waldweg 33, D-37073 Göttingen, Germany.
    • ,
  • Martin Bendszus

      Affiliations

    • Department of Neuroradiology, Julius-Maximilians-Universität, Josef-Schneider-Str. 11, 97080 Würzburg, Germany
    • ,
  • Christoph Kleinschnitz

      Affiliations

    • Department of Neurology, Julius-Maximilians-Universität, Josef-Schneider-Str. 11, 97080 Würzburg, Germany
    • ,
  • Guido Stoll

      Affiliations

    • Department of Neurology, Julius-Maximilians-Universität, Josef-Schneider-Str. 11, 97080 Würzburg, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49 931 20123769; fax: +49 931 20123489.

Received 17 August 2005; accepted 15 November 2005. published online 28 December 2005.

Abstract 

Superparamagnetic iron oxide (SPIO/USPIO) particles are a promising new tool to label cells for in vivo monitoring of their migration into the nervous system by magnetic resonance imaging (MRI). Upon systemic application, SPIO/USPIO particles are preferentially internalized by macrophages. It is unclear whether this affects their immunological profile. We tested the cytokine production of rat and mouse macrophages in vitro and found that internalization of SPIO/USPIO shifted macrophages towards an anti-inflammatory, less responsive phenotype by enhancing interleukin (IL)-10 and inhibiting tumor necrosis factor (TNF)-α production. During macrophage interaction with T-cells IL-12p40 secretion was inhibited. Based on our in vitro findings, potential immunomodulatory effects of SPIO/USPIO particles in vivo warrant further investigation.

Keywords: SPIO, USPIO, Cytokines, Macrophages, Magnetic resonance imaging

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PII: S0165-5728(05)00495-9

doi:10.1016/j.jneuroim.2005.11.011

Journal of Neuroimmunology
Volume 173, Issue 1 , Pages 166-173, April 2006

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